The future of the indigenous freshwater crayfish Austropotamobius italicus in Basque Country streams: Is it possible to survive being an inconvenient species?

The white-clawed freshwater crayfish Austropotamobius italicus is listed as “vulnerable” in the Spanish Red List of threatened species, but local legislation varies among Spanish regions. Thus, while in some places the species is classified as “in risk of extinction” and various plans of conservation and restoration have been implemented, in the Basque Country and other regions the species is not listed. The distribution of the white-clawed crayfish in the province of Biscay (Basque Country) was studied from 1993 to 2007 at more than 600 sampling locations. Results show that 108 streams were inhabited by the native crayfish species A. italicus while 137 streams were inhabited by non-native signal crayfish Pacifastacus leniusculus or red-swamp crayfish Procambarus clarkii. The spread of non-native crayfish is not the only threat to the native species whose survival is also closely dependent on how watersheds are managed. Most A. italicus populations inhabit headwaters, where forestry activities are very important. The presence of native crayfish in heavily forested areas results in a conflict of interests and makes its conservation particularly difficult. We employed a SWOT analysis – an assessment and decision tool commonly used in marketing and business – to evaluate the situation of the native white-clawed crayfish in Biscay, a province characterized by very high demographic pressure. SWOT analysis has proved to be a useful diagnostic tool and can help develop better and more accurate management strategies for the conservation of native crayfish threatened by multiple stressors

Cambios en las condiciones físicas, químicas y faunísticas de un sistema fluvial (río Oma, Bizkaia), y desaparición de una población de cangrejo autóctono (Austropotamobius pallipes) : ¿causa y/o efecto?

El Oma (cuenca del río Oka, provincia de Bizkaia) es un pequeño río relativamente poco alterado que recorre terrenos de uso forestal, agrícola y ganadero. En él habitaba una población bien establecida de cangrejo autóctono Austropotamobius pallipes, pero ésta había desaparecido en el verano de 1995, sin que se hubieran registrado mortandades masivas como las características de un episodio de afanomicosis. En este trabajo se estudia la evolución de las condiciones fisicoquímicas y de las comunidades bentónicas estivales del río Oma desde el año 1994 hasta el 2001, comparado esta dinámica respecto a las condiciones generales que presentan los hábitats de otras poblaciones de cangrejo autóctono en la red fluvial del entorno. Aparte de esta desaparición del cangrejo, también se han detectado cambios en algunas condiciones del agua así como en la composición faunística de la comunidad de macroinvertebrados. Se describen estos cambios temporales y se discuten sus posibles causas, especialmente la que ha generado la extinción de la especie protegida (influencia de depredadores, pérdida de calidad de hábitat por vertidos o lixiviados, intervenciones en cauce), pero aunque en otras cuencas cercanas se ha podido encontrar la causa principal de mortandad y/o desaparición de cangrejos, no así en el Oma, en donde podría haber tenido lugar una sinergia entre varios factores. No obstante, la evolución temporal del sistema río en los últimos años parece presentar una tendencia a recuperar las condiciones que tenía en el año 1994, tanto en la fisicoquímica como en la composición faunística.The Oma (Oka basin, province of Biscay) is a relatively little perturbed river, which runs through places where the main activities are forestry, agriculture and cattle raising. The native crayfish Austropotamobius pallipes was well established on it but in the summer of 1995 this population had disappeared. Crayfish mass mortalities that could have been due to the crayfish plague were not detected. The main objective of this work is to study the changes of abiotic and biotic summer conditions of the Oma River from 1994 to 2001. The Oma River conditions are compared with the general conditions of the close fluvial reaches inhabited by native crayfish. In addition to the disappearance of the crayfish population, changes in water chemistry and faunal composition of the benthic community were detected. Temporal variations are described in this work and the possible causes are also discussed, especially those related with the extinction of the protected species (predators' influence, decrease of the habitat quality by leaches, works on the riverbed). We have been able to detect the main cause for the mortality and/or disappearance of crayfish in other close fluvial zones, but not in the Oma River. In this stream it could have been due to the synergic interaction of several factors. However, the temporal evolution of the river system in the last years seems to show a trend to recover the conditions of the river on both physicochemical quality and faunal composition, that had in 1994

The floral biology of the olive: effect of flower number, type and distribution on fruitset

10 pages, 1 figure, 5 tables, 15 references.The effect of flower number and distribution on the fruiting behavior of various olive cultivars was studied over a period of 10 years. The number of staminate flowers within each cultivar had no significant effect on fruitset. Pre-bloom removal of up to 50% of the flowers did not affect fruitset. Variation in prebloom flower-removal position resulted in similar fruitset per inflorescence, whether flowers were removed along the inflorescence axis or from the distal half of each inflorescence. Removal of half of the inflorescences resulted in doubling the fruit set on the remaining ones, except in cv. Koronaiki which normally sets more than one fruit on most of its inflorescences. The distal fruitful inflorescence set more than one fruit (mostly two) on 70–80% of the shoots of various cultivars. In cv. Santa Caterina a clear increase in fruitset per shoot was observed when 80% of the flowers per inflorescence were removed. In this cultivar the lateral flowers were significantly more fruitful than the king flower. This however, was not the case with cv. Manzanillo.Peer reviewe

Análisis de aceituna intacta mediante espectroscopia en el infrarrojo cercano (NIRS): una herramienta de utilidad en programas de mejora de olivo

The objective of this study was to evaluate the potential use of Near-Infrared Spectroscopy (NIRS) for the analysis of oil content, moisture and fatty acids composition in intact olive fruit. A total of 287 samples, each from a single plant from an olive breeding program, were scanned by NIRS between 400 and 1700 nm. Partial least squares (PLS) regression was used to create calibration models (with 70 % of samples) relating laboratory reference values to spectral data (original, first and second derivative spectral data). The best equations obtained were validated (with 30% of samples) showing values of r2 of 0.88 % for the moisture, 0.83 % for oil content, 0.77 % for oleic acid content and 0.81 % for linoleic acid content. Therefore a reliable and accurate preselection can be made by using NIRS for both oil content and oleic acid content, with a nondestructive analysis, in a few seconds and without use neither production of chemical reagents.El objetivo de este trabajo es evaluar el potencial de la tecnología NIRS para el análisis del contenido de aceite, humedad y composición de ácidos grasos en aceituna intacta. A un total de 287 muestras de aceituna, cada una de una planta procedente de un programa de mejora de olivo, se les determinó sus datos espectroscópicos mediante reflectancia (400-1700 nm). A partir de los datos espectroscópicos originales, primera y segunda derivadas se obtuvieron diferentes ecuaciones de calibración (con el 70 % de las muestras) mediante regresión por mínimos cuadrados parciales (PLS) establecidas entre los datos espectroscópicos y los datos de laboratorio de referencia. Las mejores ecuaciones obtenidas fueron validadas (con el 30 % de las muestras) mostrando valores de r2 de 0.88 % para la humedad, 0.83 % para contenido graso, 0.77 % para contenido de ácido oleico y 0.81% para contenido de ácido linoleico. Por tanto, la tecnología NIRS puede ser de utilidad para preseleccionar genotipos por su contenido de aceite y ácido oleico con suficiente precisión y fiabilidad, sin destrucción de muestra, de forma instantánea y sin utilización ni producción de residuos químico

Perfil fenólico de aceites de oliva vírgenes obtenidos de selecciones avanzadas en un programa de mejora

The evaluation of the phenolic composition in advanced selections in breeding programs constitutes the first approach for selecting genotypes with improved olive oil quality. In this work, the influence of genotype and ripening index on the phenolic profile of olive oils from advanced selections in comparison to their genitors was studied. Fruit samples were collected in genotypes from crosses between ‘Arbequina’ × ‘Picual’, ‘Picual’ × ‘Arbequina’ and ‘Frantoio’ × ‘Picual’ at five dates from 1st October to 26th November 2009. Characterization of the phenolic profile was performed by liquid–liquid extraction with 60:40 (v/v) methanol–water and subsequent chromatographic analysis with absorption and fluorescence detection in a sequential configuration. A dual effect of genotype and fruit ripening on the phenolic profile has been observed with more pronounced genetic influence in both total (34.73% and 20.45%, respectively) and individual phenols (16.99% to 49.25% and 1.58% to 23.77%, respectively). A higher degree of variability between genotypes at early ripening stages was also observed (p<0.05). The obtained results also allow identification of selections with high content of total and individual phenols. These results suggest a strategy based on early harvesting of fruits (at the first three ripening indexes) for better comparison and selection of genotypes in further crosses in olive breeding programs aiming at improving the quality of virgin olive oil.La evaluación de la composición fenolica en selecciones avanzadas en programas de mejora constituye el primer paso para la selección de genotipos cuyos aceites son de mejor calidad. Se ha estudiado la influencia del genotipo y del índice de madurez en el perfil fenólico del aceite de oliva de selecciones avanzadas en comparación con sus genitores. Se recogieron muestras de genotipos obtenidos de cruzamientos entre 'Arbequina' × 'Picual', 'Picual' × 'Arbequina' y 'Frantoio' × 'Picual' en cinco fechas entre el 1 de octubre y el 26 noviembre de 2009. Se realizó la caracterización del perfil fenólico por extracción líquido–líquido con metanol–agua al 60:40 (v/v) seguido de un análisis cromatográfico con detección por absorción y fluorescencia en una configuración secuencial. Los resultados obtenidos mostraron un mayor grado de variabilidad entre genotipos en las primeras etapas de maduración de los frutos (p<0.05), así como el efecto de la madurez de los frutos y del genotipo en el perfil fenólico, con una influencia genética más pronunciada tanto para fenoles totales (34.73% and 20.45%, respectivamente) como individuales (entre 16.99% y 49.25% y entre 1.58% y 23.77%, respectivamente). Los resultados obtenidos han permitido la identificación de selecciones con alto contenido en fenoles totales e individuales. Ambos resultados sugieren una estrategia basada en la evaluación de los fenoles totales e individuales en las primeras etapas de la maduración para comparar y seleccionar genotipos en programas de mejora por cruzamiento encaminados a mejorar la calidad del aceite de oliva virgen

Hedgerow olive orchards: reality or utopia?

Actualmente las plantaciones de olivar tienen unas densidades que no suelen superar los 330 olivos por hectárea. El mayor coste su cultivo se produce en la recolección, donde se necesita una gran cantidad de mano de obra. Recientemente ha surgido un nuevo tipo de plantaciones cuyas densidades se acercan a las 2000 plantas por hectárea y donde los olivos se disponen en setos continuos separados unos 3,5 m. La mayor ventaja de este tipo de plantaciones es su recolección totalmente mecanizada, mediante máquinas cabalgadoras que pasan por encima de los setos, en un sistema muy parecido al usado en la vid. Además, estas plantaciones son de muy temprana entrada en producción y muy productivas, al menos en los primeros años. La variedad que se utiliza, casi en exclusiva, para este tipo de plantaciones es ‘Arbequina’. En el presente trabajo se describen los resultados de un ensayo comparativo de variedades que se estableció en el año 2000 y del que se han recogido ya 4 cosechas. Este ensayo incluye las variedades ‘Arbequina’, ‘Arbequina i-18’, ‘Arbosana’, ‘Koroneiki’, ‘Fs-17’ y ‘UC 2-35’. De ellas, ‘Arbequina’ y ‘Arbosana’ han sido las más productivas y ‘UC 2-35’ la menos vigorosa de las ensayadas. También se describen los resultados de un ensayo comparativo de densidades desde 754 a 2580 plantas/ha que fue establecido en el año 1999. Hasta ahora, la densidad más productiva parece ser la de 2000 plantas/ ha. Habrá que esperar a los próximos años para tener resultados más definitivos sobre éstos ensayos ya que es de esperar que a partir de ahora es cuando aparezcan los problemas relacionados con la competencia entre árboles. En conclusion, en un escenario donde las ayudas de la Unión Europea van a ir disminuyendo progresivamente, y donde la mano de obra es cada vez más difícil de encontrar, la capacidad real de este tipo de plantaciones de ser rentables a largo plazo determinará el futuro éxito de las mismas.Nowadays, olive orchards have a densities not higher than 330 trees/ha. The major cost correspond to harvest operations, which is very labour demanding. Recently, a new type of olive plantations with densities around 2.000 tress/ha has appeared. The major advantage of this type of plantations is their totally mechanised harvest, by vineyards straddle-harvesting machines.These plantations have a very early bearing and very productive, at least in the first years. The most common cultivar used is ‘Arbequina’. In the present work, data of the first four harvest of a variety comparative trial are reported. This trial include ‘Arbequina’, ‘Arbequina i-18’, ‘Arbosana’, ‘Koroneiki’, ‘Fs- 17’ y ‘UC 2-35’ cultivars. From the ‘Arbequina’ and ‘Arbosana’ had been the most productive and ‘UC 2-35’ the less vigorous. Results of a trial testing densities between 780 and 2580 trees/ha is also described. Up to now, the higher densities have been the most productive ones. However, more definitive results can be obtained in the coming years, when problems of competence among trees started to appear. In summary, as in the near future is expected that the subsides from the EU are going to be greatly reduced and the labour for harvesting is difficult to find, the ability to this type of plantations to be profitable at long term will determine their success

Estimating biophysical and geometrical parameters of grapevine canopies ('Sangiovese') by an unmanned aerial vehicle (UAV) and VIS-NIR cameras

Three zones of different vine vigour were identified in a mature vineyard (Vitis vinifera 'Sangiovese') to test the potential of the Visible-Near Infrared (VIS-NIR) spectral information acquired from an unmanned aerial vehicles (UAV) in estimating the leaf area index (LAI), leaf chlorophyll, pruning weight, canopy height and canopy volume of grapevines. A significant linear correlation between the normalized differential vegetation index (NDVI) and LAI or between NDVI and leaf chlorophyll was found at day of the year (DOY) 162 and 190, whereas in August the relationship between NDVI and leaf chlorophyll was less evident. The canopy volume of low-vigour (LV) vines was 35 and 45 % of the high-vigour (HV) and medium-vigour (MV) ones, respectively. The pruning weight was linearly correlated with NDVI values of each vigour cohort. A good correlation between the measured canopy volume and UAV-estimated one as well as between measured and estimated canopy height was found. Our results indicated that the combined use of VIS-NIR cameras and UAV is a rapid and reliable technique to determine canopy structure and LAI of grapevine

Assessment of a Chain Mower Performance for Weed Control under Tree Rows in an Alley Cropping Farming System

In the area under tree rows of alley cropping systems, coarse plant material as well as pruning material or stones may be present, so the use of a mower equipped with chains as cutting a tool could be advantageous. A mower designed for under-row weed control in orchards, equipped with an automatic tree-skipping mechanism, was modified by replacing blades with chains with the aim of evaluating its performance in an alley cropping system. A first trial was carried out in an open field to preliminarily compare the chain mower with the version equipped with blades in relation to different settings of working speed (1.6 and 2.4 km center dot h(-1)) and rotation speed of the cutting tool (1830 and 2500 rpm). Weed biomass reduction, weed cover reduction, weed height reduction, weed biomass regrowth, and clipping size were assessed. In a second trial, the performance of the mowers with different setting configurations was assessed in an alley cropping system under a more critical environmental condition for mowing, i.e., the presence of dew. Weed biomass reduction, weed cover reduction, weed height reduction, and the mowers' field capacity with different working speed settings were assessed. No major differences emerged between the mowers and the chain mower performance was comparable to that of the standard blade mower. The setting with the high working speed and high rotation speed of the cutting tool turns out to be the best compromise, obtaining a weed biomass reduction of 59.6%, a weed cover reduction of 40.9%, and a higher field capacity compared to the setting with the low working speed, with an increase of 47.9%